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lambda phage phosphatase λpp storage buffer  (New England Biolabs)


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    New England Biolabs lambda phage phosphatase λpp storage buffer
    Lambda Phage Phosphatase λpp Storage Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 2837 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+phosphatase+%CE%BBpp+buffer/pmc05866554-570-13-27?v=New+England+Biolabs
    Average 97 stars, based on 2837 article reviews
    lambda phage phosphatase λpp storage buffer - by Bioz Stars, 2026-06
    97/100 stars

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    lambda phage phosphatase λpp storage buffer  (New England Biolabs)
    97
    New England Biolabs lambda phage phosphatase λpp storage buffer
    Lambda Phage Phosphatase λpp Storage Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+phosphatase+%CE%BBpp+buffer/pmc05866554-570-13-27?v=New+England+Biolabs
    Average 97 stars, based on 1 article reviews
    lambda phage phosphatase λpp storage buffer - by Bioz Stars, 2026-06
    97/100 stars
    		  Buy from Supplier
    lambda phosphatase λpp buffer  (New England Biolabs)
    97
    New England Biolabs lambda phosphatase λpp buffer
    ( A ) 2D gel western blots (αGFP) of <t>lambda</t> <t>phosphatase</t> <t>(λPP)-treated</t> protein extracts from adult soil-grown plants expressing bZIP63-GFP (ox#3). ( B ) Phos-tag gel western blots showing the in vivo phosphorylation state of bZIP63 in seedlings after 6 hr extended night in the presence (+) or absence (−) of 1% sucrose and in 5 week-old soil-grown plants after 6 hr light (L) or extended night (EN). Plants either expressed 35S::bZIP63-GFP (ox#3) or a genomic fragment of bZIP63 (GY11) with a YFP-tag. Recombinant bZIP63-YFP was used as a nonphosphorylated control. Numbered arrowheads on the right mark the position of each observed bZIP63 band for easy reference with other figures (see for a comparative image of all Phos-tag western blots). For more information on the genomic line see and . ( C ) Identification of in vivo phosphorylation sites by immunoprecipitation (IP) and tandem mass spectrometry (MS/MS). An exemplary western blot of the IP is shown. The scheme at the bottom shows the positions of the identified in vivo phosphorylation sites and the total sequence coverage reached with each proteolytic enzyme (grey bars). Asterisks mark the identification of a phospho-site. For more information on samples and (phospho-) peptides see . IEF, isoelectric focusing; CBB, coomassie brilliant blue; BD, basic domain; ZIP, leucine zipper; LTQ, linear ion trap quadrupole; chym., chymotrypsin. DOI: http://dx.doi.org/10.7554/eLife.05828.012
    Lambda Phosphatase λpp Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+phosphatase+%CE%BBpp+buffer/pmc04558565-617-4-8?v=New+England+Biolabs
    Average 97 stars, based on 1 article reviews
    lambda phosphatase λpp buffer - by Bioz Stars, 2026-06
    97/100 stars
    		  Buy from Supplier
    λ protein phosphatase λpp buffer  (New England Biolabs)
    97
    New England Biolabs λ protein phosphatase λpp buffer
    ( A ) 2D gel western blots (αGFP) of <t>lambda</t> <t>phosphatase</t> <t>(λPP)-treated</t> protein extracts from adult soil-grown plants expressing bZIP63-GFP (ox#3). ( B ) Phos-tag gel western blots showing the in vivo phosphorylation state of bZIP63 in seedlings after 6 hr extended night in the presence (+) or absence (−) of 1% sucrose and in 5 week-old soil-grown plants after 6 hr light (L) or extended night (EN). Plants either expressed 35S::bZIP63-GFP (ox#3) or a genomic fragment of bZIP63 (GY11) with a YFP-tag. Recombinant bZIP63-YFP was used as a nonphosphorylated control. Numbered arrowheads on the right mark the position of each observed bZIP63 band for easy reference with other figures (see for a comparative image of all Phos-tag western blots). For more information on the genomic line see and . ( C ) Identification of in vivo phosphorylation sites by immunoprecipitation (IP) and tandem mass spectrometry (MS/MS). An exemplary western blot of the IP is shown. The scheme at the bottom shows the positions of the identified in vivo phosphorylation sites and the total sequence coverage reached with each proteolytic enzyme (grey bars). Asterisks mark the identification of a phospho-site. For more information on samples and (phospho-) peptides see . IEF, isoelectric focusing; CBB, coomassie brilliant blue; BD, basic domain; ZIP, leucine zipper; LTQ, linear ion trap quadrupole; chym., chymotrypsin. DOI: http://dx.doi.org/10.7554/eLife.05828.012
    λ Protein Phosphatase λpp Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+phosphatase+%CE%BBpp+buffer/pmc02950577-148-5-10?v=New+England+Biolabs
    Average 97 stars, based on 1 article reviews
    λ protein phosphatase λpp buffer - by Bioz Stars, 2026-06
    97/100 stars
    		  Buy from Supplier

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    ( A ) 2D gel western blots (αGFP) of lambda phosphatase (λPP)-treated protein extracts from adult soil-grown plants expressing bZIP63-GFP (ox#3). ( B ) Phos-tag gel western blots showing the in vivo phosphorylation state of bZIP63 in seedlings after 6 hr extended night in the presence (+) or absence (−) of 1% sucrose and in 5 week-old soil-grown plants after 6 hr light (L) or extended night (EN). Plants either expressed 35S::bZIP63-GFP (ox#3) or a genomic fragment of bZIP63 (GY11) with a YFP-tag. Recombinant bZIP63-YFP was used as a nonphosphorylated control. Numbered arrowheads on the right mark the position of each observed bZIP63 band for easy reference with other figures (see for a comparative image of all Phos-tag western blots). For more information on the genomic line see and . ( C ) Identification of in vivo phosphorylation sites by immunoprecipitation (IP) and tandem mass spectrometry (MS/MS). An exemplary western blot of the IP is shown. The scheme at the bottom shows the positions of the identified in vivo phosphorylation sites and the total sequence coverage reached with each proteolytic enzyme (grey bars). Asterisks mark the identification of a phospho-site. For more information on samples and (phospho-) peptides see . IEF, isoelectric focusing; CBB, coomassie brilliant blue; BD, basic domain; ZIP, leucine zipper; LTQ, linear ion trap quadrupole; chym., chymotrypsin. DOI: http://dx.doi.org/10.7554/eLife.05828.012

    Journal: eLife

    Article Title: SnRK1-triggered switch of bZIP63 dimerization mediates the low-energy response in plants

    doi: 10.7554/eLife.05828

    Figure Lengend Snippet: ( A ) 2D gel western blots (αGFP) of lambda phosphatase (λPP)-treated protein extracts from adult soil-grown plants expressing bZIP63-GFP (ox#3). ( B ) Phos-tag gel western blots showing the in vivo phosphorylation state of bZIP63 in seedlings after 6 hr extended night in the presence (+) or absence (−) of 1% sucrose and in 5 week-old soil-grown plants after 6 hr light (L) or extended night (EN). Plants either expressed 35S::bZIP63-GFP (ox#3) or a genomic fragment of bZIP63 (GY11) with a YFP-tag. Recombinant bZIP63-YFP was used as a nonphosphorylated control. Numbered arrowheads on the right mark the position of each observed bZIP63 band for easy reference with other figures (see for a comparative image of all Phos-tag western blots). For more information on the genomic line see and . ( C ) Identification of in vivo phosphorylation sites by immunoprecipitation (IP) and tandem mass spectrometry (MS/MS). An exemplary western blot of the IP is shown. The scheme at the bottom shows the positions of the identified in vivo phosphorylation sites and the total sequence coverage reached with each proteolytic enzyme (grey bars). Asterisks mark the identification of a phospho-site. For more information on samples and (phospho-) peptides see . IEF, isoelectric focusing; CBB, coomassie brilliant blue; BD, basic domain; ZIP, leucine zipper; LTQ, linear ion trap quadrupole; chym., chymotrypsin. DOI: http://dx.doi.org/10.7554/eLife.05828.012

    Article Snippet: 4 ml of 1× lambda phosphatase (λPP) buffer (NEB, Frankfurt am Main, Germany), including cOmplete protease inhibitor (Roche, Vienna, Austria), were added to 2 ml frozen and ground plant material, followed by vortexing and centrifugation.

    Techniques: Two-Dimensional Gel Electrophoresis, Western Blot, Expressing, In Vivo, Phospho-proteomics, Recombinant, Control, Immunoprecipitation, Mass Spectrometry, Tandem Mass Spectroscopy, Sequencing